Comments on Delanghe and Joyner's editorial "testing for recombinant human erythropoietin". Preanalytical and analytical issues in indirect hematological testing.
نویسندگان
چکیده
TO THE EDITOR: In their recent editorial on the disappointing disagreement between two World Anti-Doping Agency (WADA)-accredited laboratories evaluating samples for detecting recombinant human erythropoietin (Epo) in urine, Delanghe and Joyner (4) concluded that blood-based indirect Epo tests offer an interesting alternative (2). This is ideally true, considering that the urine test is proven unreliable (4). However, indirect testing, which is designed to establish an individual hematological profile (the “hematological passport”), is a hard task to accomplish. Under ideal conditions (centralized phlebotomy, standardized and traceable procedures for handling and storage of the specimens, adherence to rigorous programs for internal and external quality assessment), laboratory tests are still intrinsically biased by a certain degree of preanalytical and analytical variability (3). In the athletic field, such biases are enormously amplified and hardly governable. Strenuous physical exercise, temperature, and humidity all have substantial influences on several hematological parameters (1). Some hematological markers are also liable; this might be an additional problem when samples are collected under various environmental and technical conditions, stored and shipped for miles to distant accredited laboratories (1, 3). Finally, longitudinal comparison of several hematological variables, especially hematocrit (Ht) and hemoglobin (Hb), lacks of clinical significance if various analytical technologies are used by different accredited laboratories (i.e., Siemens ADVIA hematological analyzers substantially overestimate Hb and Ht compared with instruments from other manufacturers, such as Sysmex and Coulter; Ref. 5). When left uncontrolled and unstandardized, all these variables would dramatically affect the reliability of indirect hematological testing to detect cheating.
منابع مشابه
Comments on Delanghe and Joyner's editorial "testing for recombinant human erythropoietin". The importance of a strong collaboration between pharmaceutical companies and WADA-accredited laboratories.
TO THE EDITOR: In their recent editorial on the disappointing disagreement between two World Anti-Doping Agency (WADA)-accredited laboratories evaluating samples for detecting recombinant human erythropoietin (Epo) in urine, Delanghe and Joyner (4) concluded that blood-based indirect Epo tests offer an interesting alternative (2). This is ideally true, considering that the urine test is proven ...
متن کاملComments on Delanghe and Joyner’s Editorial “Testing for recombinant human erythropoietin”
TO THE EDITOR: In their recent editorial on the disappointing disagreement between two World Anti-Doping Agency (WADA)-accredited laboratories evaluating samples for detecting recombinant human erythropoietin (Epo) in urine, Delanghe and Joyner (4) concluded that blood-based indirect Epo tests offer an interesting alternative (2). This is ideally true, considering that the urine test is proven ...
متن کاملTesting for recombinant erythropoietin.
Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEpo. Both direct tests (indicating the presence of exogeneous Epo isoforms) and indirect tests (indicating hematological changes induced by ex...
متن کاملTesting for recombinant human erythropoietin.
ERYTHROPOIETIN (Epo) may have effects on exercise capacity and physiological regulation beyond a simple increase in red cell mass and the associated improvement in oxygen transport (4). In the context of a larger study on this topic, Lundby and colleagues (11) also asked questions about the reliability of urine testing for recombinant human Epo (rHuEpo). They studied eight healthy male subjects...
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Recombinant human erythropoietin is a glycoprotein hormone that stimulates erythropoiesis. In clonogenic assays of hematopoietic progenitors, high concen-trations of erythropoietin (Epo) increase CFU-E and diminish the number of granulocytes formed per culture plate. Fetal progenitors are more sensitive to these effects of Epo than progenitors from adults. We administered doses 50, 100, 200 IU/...
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ورودعنوان ژورنال:
- Journal of applied physiology
دوره 105 6 شماره
صفحات -
تاریخ انتشار 2008